Effective management of pH value is crucial in ensuring the normal growth, reproduction, and metabolism of microorganisms during microbial cultivation. Firstly, it is necessary to start with the formulation of the culture medium, select buffer substances and nutrients based on microbial characteristics, in order to establish a suitable pH value foundation. The application of buffering agents such as phosphates can effectively stabilize the pH value of the culture medium. Secondly, it is necessary to closely monitor the pH changes during the cultivation process. Once deviations from the appropriate range are found, they should be adjusted in a timely and appropriate manner by adding acid and alkali, but attention should be paid to their potential impact on ion concentration and microbial growth. In addition, adding nutrients through flow is also an effective strategy, such as using physiological acidic ammonium salts to regulate pH. For large-scale cultivation, it is recommended to use an automatic control system to achieve real-time monitoring and precise adjustment of pH value.

For pH regulation during microbial cultivation, the R&D team of Changsha Sichen Instrument Technology Co., Ltd. has developed the latest third-generation high-precision pH automatic control system (main models include SC-100A plus version and scientific research SC-100A Max version or SC-200A plus and scientific research SC-200A Max version). This system adopts a touch screen operation and is a new type of automated control system that integrates real-time online display, measurement, and automatic addition (acid/alkali) liquid adjustment of pH value. (For detailed information, please log in.) http://www.csscyq.com/ph.html ）At present, these models are widely used in relevant laboratories of domestic and foreign research institutes, universities, and biological enterprises.