The activity of carbonyl reductase under different pH conditions is as follows

Rhodosporidium sp. 0503 strain: This strain exhibits high catalytic activity over a wide pH range (6.0-10.0), with an optimal catalytic temperature of 40 ℃.

Rhodococcus faecalis: The carbonyl reductase isolated from Rhodococcus faecalis is relatively stable at pH 7-9, with an optimal reaction pH of 8 and an optimal temperature of 37 ℃.

Microbacterium sp.: The carbonyl reductase in this bacterium has an optimal reaction pH of 7.0 and is sensitive to changes in environmental pH. It is relatively stable between pH 6.0 and 8.0.

These pieces of information indicate that the optimal pH and stability range of carbonyl reductases vary among different strains, but most carbonyl reductases exhibit high catalytic activity over a wide pH range.

In response to the pH changes of different strains mentioned above, how to control the pH value of the strains within a stable range during the research and development stage? Sichen Instrument has developed and designed a complete set of equipment to control the pH changes of different strains; Instrument and equipment name: pH automatic control liquid addition system, its models include: SC-100A SC-200A SC-100A plus SC-100A Max SC-200A plus SC-200A Max SC-300A plus SC-300A Max SC-400A plus SC-400A Max SC-500A plus SC-500A plus SC-500A Max SC-600A plus SC-600A Max, Different models can meet different flow and pH control requirements;

The technical parameters of the pH automatic control liquid addition system are as follows

1. Working power supply: AC220V ± 10% 50Hz

2. Control mode: stacked PLC

3. Control range: 0-14pH

4. Measurement accuracy: ± 0.05pH

5. Resolution: 0.01pH

6. Pump head speed: 0.1-300rpm (arbitrary setting)

7. Display: 7-inch touch screen

8. Liquid addition rate: 0.12-190ml/min

9. Temperature compensation: automatic temperature compensation (optional)

10. PH upper and lower limits: freely set

11. PH electrode suitable temperature: 0-80 ℃ (optional high temperature up to 135 ℃)

12. PH standard buffer powder: 4.01/6.86/9.18

13. Dimensions: 385 * 340 * 210mm

14. Environmental temperature: Room temperature~45 ℃

15. Relative humidity:< 80%

16. Whole machine weight: approximately 8kg

No matter what strain, in